Modifying Enzymes
New England Biolabs, Inc. Micrococcal Nuclease – 320000 gel units
Micrococcal nuclease is derived from Staphylococcus aureus and is a relatively non-specific endo-exonuclease. It is purified from a recombinant E. coli strain that digests double-stranded, single-stranded, circular and linear nucleic acids. The enzyme is active in the pH range of 7.0 - 10.0, with optimal activity at pH 9.2 for both RNA and DNA substrates. Cleavage preferences have been observed at sites rich in adenylate, deoxyadenylate or thymidylate. Both DNA and RNA are degraded to 3 phosphomononucleotides and dinucleotides. Micrococcal Nuclease works well with the SimpleChIP Enzymatic Chromatin IP Kit (Agarose Beads) from Cell Signaling Technology.
Bio IVT CYP3A7 Reductase Bactosomes, Human rCYP Enzyme, 1 nmol/vial
CYP3A7 Reductase Bactosomes, Human rCYP Enzyme, 1 nmol/vial
Enzo Life Sciences Bio 17 ATP (250 nmol)
Alternative Name: Biotin-17-adenosine-5'-triphosphate. Formulation: Liquid. Solution in water. Purity: ≥93% (HPLC). Appearance: Clear, colorless liquid. Shipping: Shipped on Dry Ice. Long Term Storage: -20°C. Handling: Avoid freeze/thaw cycles.
ACROBiosystems GENIUS™Nuclease DMF Filed
GENIUS™Nuclease is a recombinant form of Serratia macescens extracellular endonuclease produced in Escherichia coli cells using a proprietary process at ACRObiosystems. GENIUS™Nuclease is a homodimer with monomer molecular masses about 30 kDa. Two disulfide bonds found in the nuclease are crucial to its activity and stability. The enzyme is a non-specific nuclease with high specific activity, which degrades both single- and double-stranded nucleic acids in any form ( single stranded, double stranded, linear, circular and supercoiled ). It hydrolyzes internal phosphodiester bonds present between the nucleotides to 5‘- phosphorylated oligonucleotides of 3-8 bases in length.
New England Biolabs, Inc. EnGen Spy Cas9 HF1 - 2500 pmol
EnGen Spy Cas9 HF1 is a high-fidelity, quadruple substitution (N497A/R661A/Q695A/Q926A) variant of EnGen Spy Cas9 NLS from Streptococcus pyogenes with reduced non-specific DNA cleavage. Spy Cas9 is an RNA-guided endonuclease that catalyzes site-specific cleavage of double stranded DNA. The single guide RNA (sgRNA) targets Cas9 to the region immediately upstream of a 5'-NGG-3' protospacer adjacent motif (PAM) producing a double stranded break 3 bases upstream of the PAM. EnGen Spy Cas9 HF1 contains Simian virus 40 (SV40) T antigen nuclear localization sequence (NLS) on the N- and C-termini of the protein.
New England Biolabs, Inc. DNase I-XT-5000 units
An engineered variant of DNase I DNase I-XT is a salt-tolerant DNA endonuclease that nonspecifically cleaves DNA to release di- tri- and oligonucleotide products with 5 prime phosphorylated and 3 prime hydroxylated ends. DNase I-XT acts on single- and double-stranded DNA chromatin and the DNA strand of RNADNA hybrids. While DNase I (M0303) is inhibited by salt concentrations greater than 50 mM DNase I-XT exhibits optimal activity between 50-100 mM salt and retains 65% and about 40% activity in 200 and 300 mM salt respectively. This increased salt tolerance makes DNase I-XT the preferred enzyme for DNA template removal from an in vitro transcription (IVT) reaction. Importantly DNase I-XT is RNase-free allowing for the complete removal of DNA from RNA preparations while maintaining RNA integrity.
Med Vet International Kenalog Injection (Generic Triamcinolone Acetonide), 40mg/mL, 10mL, Each
VET LICENSE NEEDS TO BE PROVIDED IN 3-4 BUSINESS DAYS OR ORDER WILL BE CANCELLED
Because Kenalog-40 Injection is a suspension, it should not be administered intravenously. This is suitable for intramuscular and intra-articular use only. 10mL per bottle
LGC GENOMICS LLC (Lucigen) Ampligase DNA Ligase Kit, 1,000 U @ 5 U/LHighly thermostable DNA ligase for applications where ligation at high temperature is beneficial or required.
Ampligase Thermostable DNA Ligase catalyzes NAD-dependent ligation of adjacent 3-hydroxylated and 5-phosphorylated termini in duplex DNA structures that are stable at high temperatures
New England Biolabs, Inc. dam Methyltransferase – 500 units
dam Methyltransferase modifies the adenine residue (N6) of the sequence GATC.
Crescent Chemical Co Inc CATALASE FROM BOVINE LIVER
Catalase from bovine liver ca. 11 000 U/mg lyophil. salt-free Homogeneous in SDS-PAGE. Ca. 40 000 U/mg protein. Size - 1G Storage Conditions - -15 °C TO -25 °C Catalog Number - 26910.02 CAS 2593710
New England Biolabs, Inc. Bacteroides Heparinase I – 600 units
Bacteroides Heparinase I, also called Heparin Lyase I, is active on heparin and the highly sulfated domains of heparan sulfate. The reaction yields oligosaccharide products containing unsaturated uronic acids which can be detected by UV spectroscopy at 232 nm.
LGC GENOMICS LLC (Lucigen) Ribonuclease R (RNase R), 250 U @ 20 U/LA 3 to 5 exoribonuclease that digests all linear RNAs except lariat or circular RNA structures. Manufactured under ISO-13485.
Ribonuclease R (RNase R) from E. coli is a magnesium-dependent 35 exoribonuclease that digests essentially all linear RNAs but does not digest lariat or circular RNA structures.Specific: Digests all linear RNAs but does not digest lariat or circular RNA structures, or double stranded RNA with 3' overhangs shorter than seven nucleotidesHeat Inactivated: Heat at 65 ¿C for 20 minutes to kill enzyme activityValuable: Use the unique properties of this exoribonuclease to study alternative splicing and gene expressionRibonuclease R includes both the enzyme and an optimised 10X Reaction Buffer.
Cayman Chemical Coenzyme Q9 10mg
A mitochondrial ubiquinone; reduces the incidence of ischemia and reperfusion-induced arrhythmia and improves post-ischemic heart rate, coronary flow, aortic flow, and left ventricular developed pressure in isolated guinea pig hearts at 5 mg/kg; reduces ischemia and reperfusion-induced myocardial infarct size and the number of apoptotic cardiomyocytes in the same model at 5 mg/kg
IBI SCIENTIFIC 3P Proteinase K Powder, 95% Purity, 100 mg
Proteinase K - 100mg IBI Proteinase K is a stable > 50mg/ml (power) highly active proteolytic enzyme that is purified from the mold Tritrachium album.